Recommended Resources
A MSU Flow Cytometry Core Facility-generated list of recommended reagents, buffers, and consumables.
FluoroFinder is a panel design app offering free accounts to academic users. The MSU Flow Cytometry Core instrument configurations are available on FluoroFinder for targeted instrument-specific panel design.
Mouse Expandable Base panel (T, B, NK, NKT, Macrophages, Monocytes, DCs and neutrophils) – for CYTEK Aurora only
A 14-color expandable panel for mouse immune cell phenotyping, enabling detailed characterization of monocytes, macrophages, NK and NKT cells, T cells, B cells, dendritic cells, and neutrophils. The panel is designed to keep channels with broad conjugate availability open, allowing seamless customization. Several strategically placed drop-in/open spots enable the addition of extra markers or conjugates, supporting straightforward expansion without disrupting the core panel design.
- Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
- Ordering information: Please contact us to order the listed reagents through a promotional bundle. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
- Product Name: Mouse Expandable Base Panel (14c)
Web Tools
BD Biosciences Spectrum Viewer
ThermoFisher Flow Cytometry Protocols
ThermoFisher offers digital and print copies of commonly used flow cytometry protocols. This is an excellent resource and a good starting place for many assays.
Cytek Cloud is an AI-equipped spectral panel design app offering free accounts to academic users (Core users, please email FACS@msu.edu to request access code).
Optimized Multicolor Immunofluorescence Panels (OMIPs)
Optimized Multicolor Immunofluorescence Panel (OMIP) is a special peer-reviewed Cytometry Part A publication type that reports on newly designed and optimized multicolor panels for flow cytometry, fluorescence microscopy, image cytometry, and other polychromatic fluorescence-based methods.
OMIPs are aimed: (1) to alleviate the development time for researchers in need of the same or highly similar panels, (2) to provide a starting point for the creation of novel OMIPs, and (3) to give the developers of the panels credit via citation or the publication.
Immunological Genome Project (ImmGen)
ImmGen is a collaborative effort between Immunologists and Computational Biologists designed with the goal of understanding immunological gene expression patterns in mice. Gene expression in various innate and adaptive immune cells can be queried within this database.
BenchSci is an AI-assisted antibody selection tool. By entering your desired antibody target into the search engine, this tool will mine publications for antibodies that have been utilized for various endpoints to aid in the reagent selection process.