Review our Sorting Best Practices, which outlines important information regarding sample preparation, recommended sample buffer, collection buffers, filtering samples, sample concentrations, and more.
Cell sorting can be performed on either our SONY MA900 (BPS, 5th floor) or BD FACSAria IIu (IQ).
Virtual training is required prior to approval of cell sorting. Typical training for cell sorting is as follows:
All Users must complete a MSU Flow Cytometry Core Cell Sorting Addendum: All materials to be sorted must be approved in your lab’s Safety Protocol within HURON Click. An addendum for projects involving sorting on a flow cytometer must be completed and approved by EH&S and/or the IBC. This addendum is required due to the increased risk of aerosolization when sorting using an electrostatic-based cell sorter. Our policies are set based upon NIH Sorter Biosafety Guidelines and ISAC (International Society for Advancement of Cytometry) Sorter Biosafety Standards. The Cell Sorting Addendum should be uploaded as an amendment to your lab’s existing HURON Click Safety Protocol. If you are a student, postdoc, or staff member, please let your PI know that this submission and approval in the HURON Click system is required prior to any approval of cell sorting by the Core.