How many cells is enough for an experiment?
How many cells do I need to stain? Adopted from: https://voices.uchicago.edu/ucflow/2020/01/16/how-many-cells-should-i-stain-the-impact-of-cell-concentration-and-antibody-concentration/ One common question we ask ourselves when starting flow cytometry is: how many cells should I stain? Keep in mind that when you digest an organ/tumor or take blood out of the animal, it would be ideal to run the entire population of cells as this is not impossible. Ideally, we…
MSU Research Featured at 2023 – Frontiers in Flow Cytometry
Exploiting Autofluorescence and Imaging Flow Cytometry to Quantitatively Phenotype Morphologically Diverse Pathogen Populations Understanding variation in pathogen virulence is a central goal of evolutionary medicine. In the shape-shifting bacterial pathogen, Pasteuria ramosa, virulence is thought to be mediated by specific bacterial morphotype(s). Accordingly, variation in the virulence of Pasteuria infections arises from variation in the absolute/relative abundance of virulence-inducing morphotypes in Pasteuria populations. Hitherto, testing…