How many cells is enough for an experiment?

How many cells do I need to stain? Adopted from: https://voices.uchicago.edu/ucflow/2020/01/16/how-many-cells-should-i-stain-the-impact-of-cell-concentration-and-antibody-concentration/ One common question we ask ourselves when starting flow cytometry is: how many cells should I stain? Keep in mind that when you digest an organ/tumor or take blood out of the animal, it would be ideal to run the entire population of cells as this is not impossible. Ideally, we…